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Sunday, July 19, 2020 | History

5 edition of Embryonic Stem Cells As a Developmental Model in Vitro (Cells Tissues Organs, Volume 165, Number 3-4, 1999) found in the catalog.

Embryonic Stem Cells As a Developmental Model in Vitro (Cells Tissues Organs, Volume 165, Number 3-4, 1999)

  • 103 Want to read
  • 31 Currently reading

Published by S Karger Pub .
Written in English

    Subjects:
  • Cellular biology,
  • Cellular physiology,
  • Developmental biology,
  • Genetic engineering,
  • Embryology,
  • Medical

  • Edition Notes

    ContributionsAnna M. Wobus (Editor), Kenneth R. Boheler (Editor)
    The Physical Object
    FormatPaperback
    Number of Pages136
    ID Numbers
    Open LibraryOL12931069M
    ISBN 103805569750
    ISBN 109783805569750
    OCLC/WorldCa76097934

    Scientists discovered ways to derive embryonic stem cells from early mouse embryos more than 30 years ago, in The detailed study of the biology of mouse stem cells led to the discovery, in , of a method to derive stem cells from human embryos and grow the cells in the laboratory. These cells are called human embryonic stem cells.   Micropatterned human embryonic stem cells (hESCs) self-organize into reproducible spatial domains corresponding to each of the germ layers and were recently established as a method to recapitulate human gastrulation in vitro (Warmflash et al., ). This system can be easily manipulated and by:

    Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. Stem Cells. ;– [35] Gerecht-Nir S, Ziskind A, Cohen S, Itskovitz-Eldor J. Human embryonic stem cells as an in vitro model for human vascular development and the induction of vascular differentiation. Embryonic stem cells are grown from cells found in the embryo when it is just a few days old. In humans, mice and other mammals, the embryo is a ball of approximately cells at this stage. It is known as a blastocyst and has two parts: An outer layer of cells, or trophectoderm, will form the placenta that supports the embryo as it grows.

    Stem cells have the capacity to undergo self‐renewal and also to give rise to differentiated cells upon receiving appropriate developmental cues. The action of stem cells is crucial for proper embryonic development in vivo, and even in adult life, endogenous adult stem cells are central players in tissue homeostasis and organ : Bindu Prabhakar, Soowan Lee, Theodore P. Rasmussen. Mouse embryonic stem cells. The most-studied embryonic stem cells are mouse embryonic stem cells, which were first reported in This type of stem cell can be cultured indefinitely in the presence of leukemia inhibitory factor (LIF), a glycoprotein cultured mouse embryonic stem cells are injected into an early mouse embryo at the blastocyst stage, they will become .


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Embryonic Stem Cells As a Developmental Model in Vitro (Cells Tissues Organs, Volume 165, Number 3-4, 1999) Download PDF EPUB FB2

Irina Klimanskaya, in Principles of Regenerative Medicine (Third Edition), Introduction. Embryonic stem cells (ESC) can be viewed as an immortal extension of short-lived pluripotent cells that exist in a preimplantation embryo.

These pluripotent cells become all of the tissues of the body during embryo development, and cell lines created in vitro from these pluripotent. Irina Klimanskaya, in Principles of Regenerative Medicine (Third Edition), Introduction. Embryonic stem cells (ESC) can be viewed as an immortal extension of short-lived pluripotent cells that exist in a preimplantation embryo.

These pluripotent cells become all of the tissues of the body during embryo development, and cell lines created in vitro from these. Embryonic stem cells are derived from embryos at a developmental stage before the time that implantation would normally occur in the uterus. Fertilization normally occurs in the oviduct, and during the next few days, a series of cleavage divisions occur as the embryo travels down the oviduct and into the uterus.

Each of the cells (blastomeres. Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System drug delivery, and drug testing.

In Embryonic Stem Cells: Methods and Protocols, Kursad Turksen and a panel of international experts describe their most productive methods for using ES cells as in vitro developmental models for many cell and tissue. "A highly readable and useful book .[It] will help many to navigate the uncharted waters of human embryonic stem cell biology." (The British Society for Cell Biology Newsletter, Autumn, ) "[It] is a valuable resource for seasoned and novice researchers; it would make an excellent addition to the reference collection of any medical library or research laboratory."5/5(2).

Offers an online supplement summarizing current clinical applications of stem cells; Written by a prominent leader in the field, The Science of Stem Cells is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology.5/5(1).

Embryonic stem cells (ESCs) are pluripotent stem cells derived from a preimplantation embryo. ESCs are distinguished by two major properties: their pluripotency (the ability to differentiate into. Abstract. Embryonic stem (ES) cells differentiate efficiently in vitro and give rise to many different somatic cell types.

Hematopoietic progenitors present within differentiated ES cells (embryoid bodies, EBs) can be identified by replating EB cells into Cited by: In Embryonic Stem Cells: Methods and Protocols, Kursad Turksen and a panel of international experts describe their most productive methods for using ES cells as in vitro developmental models for many cell and tissue : Humana Press.

Roles of stem cells in toxicological studies. Stem cells may play several relevant roles in cellular assays for testing toxicity, and these roles can be played in either embryotoxicity or in organogenesis for testing toxicity in adult tissues (Figure 1).Indeed, embryonic stem cells (ESC) or reprogrammed-induced pluripotent stem cells (iPSC) can be employed through forced Cited by: 5.

Mouse and human embryonic stem cells. The first forays into understanding pluripotency in the mammalian embryo began with rather simple experiments in which pieces of mouse germ cell tumors known as teratocarcinomas were grown in culture and a variety of pluripotent cell types emerged (Solder, ).One cell type, termed Embryonal Carcinoma Cells (ECCs), produced Cited by: 3.

Embryonic stem cells, or ES cells, have a number of unique properties that distinguish them from regular adult cells. Therefore, scientists have devised special culturing techniques to maintain or take advantage of these properties.

When cultured properly, ES cells can divide indefinitely to make more of themselves. We sought to determine the impact of standard tobacco cigarettes and e-cigarettes on heart development in vitro and in vivo. Methods Zebrafish (Danio rerio) were used to assess developmental effects in vivo and cardiac differentiation of human embryonic stem cells (hESCs) was used as a model for in vitro cardiac by:   Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro.

However, the assembly system and developmental Cited by: Murine embryonic stem (ES) cells represent a model system for studying certain aspects of hemopoiesis because they can differentiate in vitro into several cell types, including those of the.

Human Embryonic Stem Cells in Development, Volumethe latest release in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as recapitulating pancreas development from human embryonic stem cells in a dish, modeling mammalian gastrulation with embryonic.

STEM CELLS, a peer reviewed journal published monthly, provides a forum for prompt publication of original investigative papers and concise reviews. STEM CELLS is read and written by clinical and basic scientists whose expertise encompasses the rapidly expanding fields of stem and progenitor cell biology.

STEM CELLS welcomes original articles and concise reviews. Stem cells as in vitro models for drug discovery. Drug testing using in vitro models has been a major boost, not only in identifying potential therapeutic compounds, but also in increasing our understanding of their absorption, distribution, metabolism, and excretion (ADME) properties The development of various in vitro ADME models has made a clear impact on Cited by: Embryonic stem cells (ESCs) are self‐renewing pluripotent cells derived from the inner cell mass (ICM) of blastocyst of the developing embryo.

This chapter focuses on the use of (human) pluripotent stem cells (PSCs) and their progeny to assess (developmental) neurotoxicity.

The neural tube is formed by neuroepithelial progenitor (NEP) by: 2. Vitrolife. Global Human Embryonic Stem Cells (HESC) Market Segmentation. This market was divided into types, applications and regions.

The growth of each segment provides an accurate calculation and forecast of sales by type and application in terms of volume and value for the period between and. Gastruloids are three dimensional aggregates of embryonic stem cells (ESCs) that, under appropriate culture conditions develop an embryo-like organization with three orthogonal axes and a precise distribution of the primordia for multiple derivatives of the three germ layers in the absence of extraembryonic tissues.

Significantly, they lack fore- mid- and hindbrain."Between public opinion and public policy: human embryonic stem-cell research and path-dependency." J Law Med Ethics 37(4):Leist, Marcel et al.

"The Biological and Ethical Basis of the Use of Human Embryonic Stem Cells for In Vitro Test Systems or Cell Therapy," Altex 25 (3)pp. [PDF]. Human embryonic stem (hES) cells that have been derived from the inner cell mass of preimplantation embryos represent a perfect model to mimic early human development as, like their in vivo counterparts, they can be propagated indefinitely and can be differentiated in vitro into all cells of the by: 4.